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HTTP 500 Internal Server Error when logging into Exchange 2013 SP1 /ECP

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Hi, 

So after lot of effort finally here,

We have 3 Exchange server as below

1-EXCH MAILBOX/CAS

2-CAS

3-MAILBOX 

currently all users are connected with 1-EXCH MAILBOX/CAS and wokring fine with /owa /ecp, internal and external directories.

then i decided to configure DAG with Mailbox servers, so setup mailbox and cas two addidtional servers. after setup CAS i'm trying to access new CAS server /ecp and /owa with localhost but getting HTTP 500 Internal Server Error.

Tried:

.changed framworkclr verion 2.0 but no luck

.rebuild ecp/owa virtual directories but same issue

Please suggest me further t-shoot. 

Thanks 


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